Dr Amrutha Chilumuri
MRG and Infection and Immunity, University of East London
Amyloid β (Aβ) is the major component of the senile plaques in Alzheimer’s disease (AD). The mechanism underlying cell death in AD includes oxidative stress, apoptosis, impaired mitochondrial function and receptor mediated effects. Compounds that specifically bind to Aβ are neuroprotective. The overexpression of genes such as catalase and KiSS-1 result in the production of proteins that bind to Aβ. The interaction of these proteins with Aβ was found to be protective against Aβ mediated toxicity in SH-SY5Y neuroblastoma cells. The neuroprotective protein sequence could be used to design peptides that can bind Aβ, which could be of therapeutic use in designing drugs for treating AD.
Chilumuri, A., Markiv, A., Milton, N.G.N (2014). Immunocytochemical staining of endogenous nuclear proteins with the HIS-1 anti-poly-histidine monoclonal antibody: A potential source of error in His-tagged protein detection. Acta Histochemica, 116, 1022-1028. 0.
Chilumuri, A., Odell, M. & Milton, N.G.N. (2013). Benzothiazole aniline-tetra (ethylene glycol) and 3-amino-1,2,4-triazole inhibit neuroprotection against amyloid peptides by catalase overexpression in vitro.
ACS Chem. Neurosci., 4 (11), 1501-1512.
Chilumuri, A., Odell, M. & Milton, N. (2013). The neuroprotective role of catalase overexpression in SH-SY5Y cells against beta-amyloid and H2O2 toxicity. Alzheimer's & Dementia: The Journal of the Alzheimer's Association, 9 (4 (Supplement)), P361.
Chilumuri, A. & Milton, N.G.N. (2013). The role of neurotransmitters in protection against amyloid-β toxicity by KiSS-1 overexpression in SH-SY5Y neurons. ISRN Neuroscience, 253210.
Chilumuri, A., Ashioti, M., Nercessian, A.N. & Milton, N.G.N. (2013). Immunolocalization of kisspeptin associated with amyloid-ß deposits in the pons of an Alzheimer's disease patient.
J. Neurodegen. Dis., 879710.
Milton, N.G.N., Chilumuri, A., Rocha-Ferreira, E., Nercessian, A.N. & Ashioti, M. (2012). Kisspeptin prevention of amyloid-ß peptide neurotoxicity in vitro. ACS Chem. Neurosci., 3 (9), 706-719.